He was the only son of Louis-Henri II, Duke de Bourbon, he emigrated with his father at the outbreak of the French Revolution and served in his grandfather's émigré army from 1792 until its dissolution after the Treaty of Lunéville in 1801. He secretly married Charlotte de Rohan-Rochefort and settled at Ettenheim, in Baden, just across the French border. 

In 1804 Napoleon, then first consul, received intelligence that connected him with the conspiracy to overthrow him then being planned by Cadoudal and Pichegru. The report was false, but Napoleon ordered Enghien's arrest, and French gendarmes crossed the Rhine secretly and seized him. He was brought to the castle of Vincennes near Paris, where a court-martial was hurriedly gathered to try him, and he was shot about a week after his arrest. Though his father survived him, the Duke d'Enghien was genealogically the last prince of the house of Condé. 

The indignation that the execution aroused throughout Europe provoked the often quoted and misquoted comment upon the execution, 'C'est pire qu'un crime, c'est une faute.' ('It's worse than a crime, it's a mistake.')